Device

Part:BBa_K2018035

Designed by: Joel Vej-Nielsen   Group: iGEM16_SDU-Denmark   (2016-06-24)


phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

A hybrid promoter-RBS phaCAB construct. This brick is part of a library of PHB producing cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 979
    Illegal BglII site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 1268
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 2199
    Illegal NgoMIV site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4065


T--SDU-Denmark--PHB_biobrickconstruct_Plastic.png

The table illustrates the different hybrid proter-RBS constructs that have been tested. The color gradient indicates strenght of promoter and RBS where the darkest color corresponds to the highest strength.




T--SDU-Denmark--PBH_productionRedflourescence_plastic.png

The figure illustrates the amount of plastic in strains containing the different BioBricks from the library.



T--SDU-Denmark--PHB_ren_HNMR.png

The product of the phaCAB genes has been analysed with gas chromatography and identified as PHB.


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